By Roger G. Harrison, Paul W. Todd, Scott R. Rudge, Demetri P. Petrides
Designed for undergraduates, graduate scholars, and practitioners, Bioseparations technological know-how and Engineering fills a serious want within the box of bioseparations. present, accomplished, and concise, it covers bioseparations unit operations in extraordinary intensity. In all the chapters, the authors use a constant approach to explaining unit operations, beginning with a qualitative description noting the importance and normal software of the unit operation. They then illustrate the clinical program of the operation, boost the mandatory mathematical concept, and at last, describe the purposes of the idea in engineering perform, with an emphasis on layout and scaleup. precise to this article is a bankruptcy devoted to bioseparations strategy layout and economics, during which a technique simular, SuperPro fashion designer® is used to investigate and overview the construction of 3 very important organic items.
New to this moment variation are up to date discussions of second research, machine simulation, membrane chromatography, and evaporation, between others, in addition to revised challenge units. particular beneficial properties contain easy information regarding bioproducts and engineering research and a bankruptcy with bioseparations laboratory routines. Bioseparations technological know-how and Engineering is perfect for college kids and pros operating in or learning bioseparations, and is the ultimate textual content within the box.
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Extra resources for Bioseparations Science and Engineering
Clearly, primary metabolites are the raw material for the synthesis of secondary metabolites. The primary metabolites, the level of phosphate in the culture medium, and the cell’s energy charge are important modulators of the pathways leading to secondary metabolites . Secondary metabolites made by plants and fungi have found many roles in human culture. 9 General routes of biosynthesis of secondary metabolites. The heavy arrows signify enzyme-catalyzed reaction sequences, and the products at the end of these arrows are secondary metabolites.
Specific properties of amino acid side groups can be exploited in purification methods. A protein rich in acidic or basic amino acids on its surface can be adsorbed by ion exchange or separated by electrophoresis. Many aliphatic side chains can result in preferential adsorption onto or extraction into nonpolar separation media. The free–SH (sulfhydryl) group of cysteine can be used to bind proteins to immobilized mercury. Histidine forms coordination complexes with metals, a fact that is being heavily exploited in protein purification by adsorption methods, as we shall see in Chapter 7.
Antibodies are globular proteins synthesized by B lymphocytes in animals. All B lymphocytes that are members of a “clone” produce identical antibody molecules, but different clones produce different antibodies, with a combinatorial number of possibilities, perhaps more than 108 different possible peptide sequences. How do they do that? Genes that code for proteins are supposed to be constant and stable, but antibody genes are an exception; they are susceptible to internal rearrangements of DNA base sequences that encode the “hypervariable” region of antibodies, the part that binds antigens.
Bioseparations Science and Engineering by Roger G. Harrison, Paul W. Todd, Scott R. Rudge, Demetri P. Petrides