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Get Biogenesis of Plant Cell Wall Polysaccharides PDF

By Frank Loewus

ISBN-10: 0124553508

ISBN-13: 9780124553507

ISBN-10: 0323152465

ISBN-13: 9780323152464

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3Q to 50% UDP-Mannose, UDP-Galactose. Greater than 95% Glucuronic A c i d - l - P , UDP-Glucuronic Acid. All sugars and sugar acids were D and sugar-1-phosphates were a. Sugars and sugar acids were 10 mM in the standard reaction mixture: a l l other inhibitors were 4 mM in reaction mixtures containing 1 mM ATP. 0 99 44 PLANT CELL WALL'POLYSACCHARI DES and 0,8 mM UDP-glucuronic acid. The type of inhibition was not established, but it seems likely that the former compound, a product of the enzyme, is simply binding to the substrate site.

73 ND Brassica sp. 56 The symbol ND means that activity was not detectable under the conditions of the assay. In certain instances the dehydrogenase was not assayed (--). 55 R. M. ROBERTS AND J. J. CETORELLI The absence of pyrophosphorylase in WoZ^sielZa fatotvidoLViCL is particularly significant, as this plant is also unusual in that it does not metabolize mt/O-inositol to cell-wall polysaccharide (8). Rather, it converts it to mj/o-inositol phosphates of various kinds· Presumably, in this tissue the UDP-GlcUA is synthesized from UDP-glucose, as the dehydrogenase is present in significant amounts.

M. Ray, Plant PWyhlol. 47, 537 (1971). F. Loewus, Ann. P&v. Plant PkyAtol. 11, 337 (1971). F. Loewus, Ann. M. V. Acad. Sel. 165 [1], 577 (1969). M. Kroh and F. Loewus, Sciwct 160, 1352 (1968). R. M. Roberts, J. Blol. Ck&n. 146, 4995 (1971). E. F. Neufeld, D. S. Feingold, W. Z. Hassid, Atick. Blockm. Biopky*. 83, 96 (1959); D. S. Feingold, E. F. Neufeld and W. Z. Hassid in Wodvin M&tkodb o{) Plant AnoJLybLb (H. F. Linskens, B. D. Sanwal and M. V. Tracey, eds) , Vol. VII, Springer-Verlag, Berlin (1964) p.

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Biogenesis of Plant Cell Wall Polysaccharides by Frank Loewus

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